GSM7729130: 92-1,FHT-1015,4h,ChIP-seq,SOX10; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SOX10
Cell type
Cell type Class
Others
Cell type
92-1
NA
NA
Attributes by original data submitter
Sample
source_name
92-1
cell line
92-1
cell type
uveal melanoma
chip antibody
SOX10 (Abcam cat# ab155279)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7729130
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Treated 92-1 cells were fixed with 1/10 volume freshly prepared Formaldehyde solution (37% Formaldehyde [Sigma], 5M NaCl [Sigma], 0.5 M EDTA pH 8.0 [Sigma], 1M HEPES pH 7.9 [Teknova]), and neutralized with 1/20 volumes of 2.5 M glycine (Sigma). Cells were harvested and washed twice with ice-cold PBS (ThermoFisher) containing 0.5% Igepal (Sigma). During the final wash, 1 mM PMSF was included. Cells were pelleted and following nuclei extraction, chromatin was sonicated. After centrifugating the sonicated chromatin for 10 min at 10,000 rpm, soluble fractions were incubated with the following antibodies: SMARCA4 (Abcam cat# ab110641), SOX10 (Abcam cat# ab155279), MITF (Active Motif cat# 39789), TFAP2A (Santa Cruz cat# sc-12726), H3K27ac (Active Motif cat# 39133). Illumina libraries were prepared by Active Motif using standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation according to the standard Illumina ChIP-seq library.